diff --git a/.python-version b/.python-version
new file mode 100644
index 0000000..24ee5b1
--- /dev/null
+++ b/.python-version
@@ -0,0 +1 @@
+3.13
diff --git a/README.md b/README.md
index ac7cde3..3357fc1 100644
--- a/README.md
+++ b/README.md
@@ -1,2 +1,115 @@
-# digital-embryo
+# 安装 Vite 创建工具（只需一次）
+```
+npm create vite@latest embryo-frontend -- --template react-ts
+cd embryo-frontend
+npm install
+npm install three @react-three/fiber @react-three/drei
+npm install zustand axios react-router-dom
+npm run dev
 
+cd ..
+mkdir embryo-backend
+cd embryo-backend
+```
+# 创建虚拟环境（推荐）
+安装uv：<https://docs.astral.sh/uv/getting-started/installation/>
+```
+powershell -ExecutionPolicy ByPass -c "irm https://astral.sh/uv/install.ps1 | iex"
+```
+
+# 运行
+```
+.\start-dev.ps1
+```
+<http://localhost:5000/api/gene/SOX2>
+
+
+## 脚本功能：
+
+1. **同时启动两个服务**：
+   - 前端：在 `embryo-frontend` 目录中运行 `npm run dev`
+   - 后端：使用 `uv run` 运行 `embryo-backend/app.py`
+
+2. **智能检查**：
+   - 验证目录和文件是否存在
+   - 监控服务状态
+   - 提供彩色输出和状态信息
+
+3. **优雅的停止机制**：
+   - 使用 Ctrl+C 可以同时停止两个服务
+   - 自动清理后台作业
+
+## 使用方法：
+
+在项目根目录中运行：
+
+```powershell
+.\start-dev.ps1
+```
+
+或者如果需要执行权限：
+
+```powershell
+Set-ExecutionPolicy -ExecutionPolicy RemoteSigned -Scope CurrentUser
+.\start-dev.ps1
+```
+
+## 预期输出：
+
+- 前端开发服务器通常运行在：`http://localhost:5173`
+- 后端API服务器通常运行在：`http://localhost:5000`
+
+脚本会显示启动状态，并在两个服务都启动后保持运行状态。按 Ctrl+C 可以同时停止所有服务。
+
+现在您可以使用这个脚本来快速启动整个开发环境了！
+
+
+# 20250721 3D点云可视化
+
+```
+npm install three
+```
+
+# 20250721生成假的胚胎数据：
+```
+uv run FakeEmbyro.py
+
+
+cd embryo-frontend
+npm install chart.js react-chartjs-2
+```
+
+
+
+```
+import numpy as np
+import pandas as pd
+import anndata as ad
+import os
+
+script_path = os.path.dirname(os.path.realpath(__file__))
+N = 1500
+radius = 10
+np.random.seed(42)
+
+phi = np.random.uniform(0, np.pi, N)
+theta = np.random.uniform(0, 2 * np.pi, N)
+
+x = radius * np.sin(phi) * np.cos(theta)
+y = radius * np.sin(phi) * np.sin(theta)
+z = radius * 0.7 * np.cos(phi)
+
+genes = ["SOX2", "NANOG", "POU5F1", "T", "OTX2", "ZIC2", "FOXA2", "LEFTY1"]
+expression_data = {
+    gene: np.exp(-(x**2 + y**2 + z**2) / (2 * radius**2)) + 0.1 * np.random.rand(N)
+    for gene in genes
+}
+
+obs = pd.DataFrame({"cell_id": [f"cell_{i}" for i in range(N)]}, index=[f"cell_{i}" for i in range(N)])
+var = pd.DataFrame(index=genes)
+obsm = {"spatial": np.vstack([x, y, z]).T}
+X = np.vstack([expression_data[gene] for gene in genes]).T  # shape (N, G)
+
+adata = ad.AnnData(X=X, obs=obs, var=var, obsm=obsm)
+adata.write(f"{script_path}/CS9.h5ad")
+```
diff --git a/embryo-backend/Data/CS7.h5ad b/embryo-backend/Data/CS7.h5ad
new file mode 100644
index 0000000..44fefc0
Binary files /dev/null and b/embryo-backend/Data/CS7.h5ad differ
diff --git a/embryo-backend/Data/CS8.h5ad b/embryo-backend/Data/CS8.h5ad
new file mode 100644
index 0000000..8322a51
Binary files /dev/null and b/embryo-backend/Data/CS8.h5ad differ
diff --git a/embryo-backend/Data/CS9.h5ad b/embryo-backend/Data/CS9.h5ad
new file mode 100644
index 0000000..3c849a1
Binary files /dev/null and b/embryo-backend/Data/CS9.h5ad differ
diff --git a/embryo-backend/Data/FakeEmbryo.py b/embryo-backend/Data/FakeEmbryo.py
new file mode 100644
index 0000000..0aaa749
--- /dev/null
+++ b/embryo-backend/Data/FakeEmbryo.py
@@ -0,0 +1,80 @@
+import numpy as np
+import pandas as pd
+import anndata as ad
+import os
+
+np.random.seed(42)
+
+# 参数配置
+stages = [("CS7", 500), ("CS8", 1000), ("CS9", 1500)]
+genes = ["SOX2", "NANOG", "T", "POU5F1", "OTX2", "ZIC2", "FOXA2", "LEFTY1"]
+layers = {
+    "Ectoderm": 1.0,     # 外层
+    "Mesoderm": 0.85,    # 中层
+    "Endoderm": 0.7,     # 内层
+}
+notochord_ratio = 0.05  # 脊索/原条占总细胞数的比例
+
+# 保存路径
+script_path = os.path.dirname(os.path.realpath(__file__))
+
+for stage, total_cells in stages:
+    n_noto = int(total_cells * notochord_ratio)
+    n_layer = total_cells - n_noto
+    n_per_layer = n_layer // len(layers)
+
+    positions = []
+    cell_types = []
+    ids = []
+
+    # 椭球比例参数
+    a, b, c = 10, 8, 5
+
+    for i, (layer_name, scale) in enumerate(layers.items()):
+        N = n_per_layer
+        phi = np.random.uniform(0, np.pi, N)
+        theta = np.random.uniform(0, 2*np.pi, N)
+        r = scale  # 层的半径比例
+
+        x = a * r * np.sin(phi) * np.cos(theta)
+        y = b * r * np.sin(phi) * np.sin(theta)
+        z = c * r * np.cos(phi)
+
+        for xi, yi, zi in zip(x, y, z):
+            positions.append([xi, yi, zi])
+            cell_types.append(layer_name)
+            ids.append(f"{stage}_{layer_name}_{len(ids)}")
+
+    # 添加脊索/原条样结构（Z轴中间偏下的细胞）
+    x = np.random.normal(0, 0.5, n_noto)
+    y = np.random.normal(0, 0.5, n_noto)
+    z = np.linspace(-3, 3, n_noto)
+
+    for xi, yi, zi in zip(x, y, z):
+        positions.append([xi, yi, zi])
+        cell_types.append("Notochord")
+        ids.append(f"{stage}_Noto_{len(ids)}")
+
+    # 构造表达矩阵
+    pos_arr = np.array(positions)
+    X_data = []
+    for gene in genes:
+        center = np.random.randn(3) * 2
+        spread = np.random.uniform(5, 12)
+        expr = np.exp(-np.sum((pos_arr - center)**2, axis=1) / (2 * spread**2))
+        expr += 0.05 * np.random.rand(len(pos_arr))  # 加一点噪声
+        X_data.append(expr)
+    X = np.vstack(X_data).T  # shape (cells, genes)
+
+    # 构造 AnnData
+    obs = pd.DataFrame({
+        "cell_id": ids,
+        "cell_type": cell_types,
+        "stage": stage
+    }, index=ids)
+    var = pd.DataFrame(index=genes)
+    obsm = {"spatial": pos_arr}
+
+    adata = ad.AnnData(X=X, obs=obs, var=var, obsm=obsm)
+    adata.write_h5ad(os.path.join(script_path, f"{stage}.h5ad"))
+    print(f"Saved: {stage}.h5ad with {len(ids)} cells.")
diff --git a/embryo-backend/Data/embryo_mock_3D_expression.h5ad b/embryo-backend/Data/embryo_mock_3D_expression.h5ad
new file mode 100644
index 0000000..f4cc1bb
Binary files /dev/null and b/embryo-backend/Data/embryo_mock_3D_expression.h5ad differ
diff --git a/embryo-backend/app.py b/embryo-backend/app.py
new file mode 100644
index 0000000..6bd81e4
--- /dev/null
+++ b/embryo-backend/app.py
@@ -0,0 +1,61 @@
+from flask import Flask, request, jsonify
+from flask_cors import CORS
+import anndata as ad
+import os
+
+app = Flask(__name__)
+CORS(app)
+
+# 存放所有 h5ad 文件的目录
+DATA_DIR = os.path.join(os.path.dirname(__file__), "Data")
+
+# 缓存已加载的 AnnData 对象，避免重复加载
+loaded_data = {}
+
+def load_adata(stage):
+    if stage not in loaded_data:
+        filepath = os.path.join(DATA_DIR, f"{stage}.h5ad")
+        if not os.path.exists(filepath):
+            return None
+        loaded_data[stage] = ad.read_h5ad(filepath)
+    return loaded_data[stage]
+
+@app.route("/api/genes")
+def list_genes():
+    stage = request.args.get("stage")
+    adata = load_adata(stage)
+    if not adata:
+        return jsonify({"error": f"Stage '{stage}' not found"}), 404
+    return jsonify(list(adata.var_names))
+
+@app.route("/api/gene_expression")
+def get_gene_expression():
+    gene = request.args.get("gene")
+    stage = request.args.get("stage")
+    adata = load_adata(stage)
+
+    if not adata:
+        return jsonify({"error": f"Stage '{stage}' not found"}), 404
+    if gene not in adata.var_names:
+        return jsonify({"error": f"Gene '{gene}' not found in {stage}"}), 404
+
+    # 读取表达值
+    expr = adata[:, gene].X
+    expr = expr.toarray().flatten() if hasattr(expr, "toarray") else expr.flatten()
+
+    # 读取三维坐标
+    if "spatial" in adata.obsm:
+        coords = adata.obsm["spatial"]
+    elif all(k in adata.obs for k in ("x", "y", "z")):
+        coords = adata.obs[["x", "y", "z"]].values
+    else:
+        return jsonify({"error": "No spatial coordinates found"}), 500
+
+    result = [
+        {"x": float(x), "y": float(y), "z": float(z), "value": float(v)}
+        for (x, y, z), v in zip(coords, expr)
+    ]
+    return jsonify({"gene": gene, "expression": result})
+
+if __name__ == "__main__":
+    app.run(debug=True)
diff --git a/embryo-frontend/.gitignore b/embryo-frontend/.gitignore
new file mode 100644
index 0000000..a547bf3
--- /dev/null
+++ b/embryo-frontend/.gitignore
@@ -0,0 +1,24 @@
+# Logs
+logs
+*.log
+npm-debug.log*
+yarn-debug.log*
+yarn-error.log*
+pnpm-debug.log*
+lerna-debug.log*
+
+node_modules
+dist
+dist-ssr
+*.local
+
+# Editor directories and files
+.vscode/*
+!.vscode/extensions.json
+.idea
+.DS_Store
+*.suo
+*.ntvs*
+*.njsproj
+*.sln
+*.sw?
diff --git a/embryo-frontend/index.html b/embryo-frontend/index.html
new file mode 100644
index 0000000..839e840
--- /dev/null
+++ b/embryo-frontend/index.html
@@ -0,0 +1,13 @@
+<!doctype html>
+<html lang="en">
+  <head>
+    <meta charset="UTF-8" />
+    <link rel="icon" type="image/svg+xml" href="/vite.svg" />
+    <meta name="viewport" content="width=device-width, initial-scale=1.0" />
+    <title>Vite + TS</title>
+  </head>
+  <body>
+    <div id="root"></div>
+    <script type="module" src="/src/main.tsx"></script>
+  </body>
+</html>
diff --git a/embryo-frontend/package-lock.json b/embryo-frontend/package-lock.json
new file mode 100644
index 0000000..7aa3520
--- /dev/null
+++ b/embryo-frontend/package-lock.json
@@ -0,0 +1,1443 @@
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+  "version": "0.0.0",
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+  "requires": true,
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+    "": {
+      "name": "embryo-frontend",
+      "version": "0.0.0",
+      "dependencies": {
+        "axios": "^1.10.0",
+        "chart.js": "^4.5.0",
+        "react": "^19.1.0",
+        "react-chartjs-2": "^5.3.0",
+        "react-dom": "^19.1.0",
+        "react-router-dom": "^7.6.3",
+        "three": "^0.178.0"
+      },
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+        "vite": "^7.0.4"
+      }
+    },
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+      "funding": {
+        "url": "https://github.com/sponsors/SuperchupuDev"
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+        "vite": "bin/vite.js"
+      },
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+      },
+      "funding": {
+        "url": "https://github.com/vitejs/vite?sponsor=1"
+      },
+      "optionalDependencies": {
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+        "stylus": ">=0.54.8",
+        "sugarss": "^5.0.0",
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+        "yaml": "^2.4.2"
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+        "less": {
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+        },
+        "lightningcss": {
+          "optional": true
+        },
+        "sass": {
+          "optional": true
+        },
+        "sass-embedded": {
+          "optional": true
+        },
+        "stylus": {
+          "optional": true
+        },
+        "sugarss": {
+          "optional": true
+        },
+        "terser": {
+          "optional": true
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+        "tsx": {
+          "optional": true
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+        "yaml": {
+          "optional": true
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+    }
+  }
+}
diff --git a/embryo-frontend/package.json b/embryo-frontend/package.json
new file mode 100644
index 0000000..17934c2
--- /dev/null
+++ b/embryo-frontend/package.json
@@ -0,0 +1,24 @@
+{
+  "name": "embryo-frontend",
+  "private": true,
+  "version": "0.0.0",
+  "type": "module",
+  "scripts": {
+    "dev": "vite",
+    "build": "tsc && vite build",
+    "preview": "vite preview"
+  },
+  "devDependencies": {
+    "typescript": "~5.8.3",
+    "vite": "^7.0.4"
+  },
+  "dependencies": {
+    "axios": "^1.10.0",
+    "chart.js": "^4.5.0",
+    "react": "^19.1.0",
+    "react-chartjs-2": "^5.3.0",
+    "react-dom": "^19.1.0",
+    "react-router-dom": "^7.6.3",
+    "three": "^0.178.0"
+  }
+}
diff --git a/embryo-frontend/public/vite.svg b/embryo-frontend/public/vite.svg
new file mode 100644
index 0000000..e7b8dfb
--- /dev/null
+++ b/embryo-frontend/public/vite.svg
@@ -0,0 +1 @@
+<svg xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" aria-hidden="true" role="img" class="iconify iconify--logos" width="31.88" height="32" preserveAspectRatio="xMidYMid meet" viewBox="0 0 256 257"><defs><linearGradient id="IconifyId1813088fe1fbc01fb466" x1="-.828%" x2="57.636%" y1="7.652%" y2="78.411%"><stop offset="0%" stop-color="#41D1FF"></stop><stop offset="100%" stop-color="#BD34FE"></stop></linearGradient><linearGradient id="IconifyId1813088fe1fbc01fb467" x1="43.376%" x2="50.316%" y1="2.242%" y2="89.03%"><stop offset="0%" stop-color="#FFEA83"></stop><stop offset="8.333%" stop-color="#FFDD35"></stop><stop offset="100%" stop-color="#FFA800"></stop></linearGradient></defs><path fill="url(#IconifyId1813088fe1fbc01fb466)" d="M255.153 37.938L134.897 252.976c-2.483 4.44-8.862 4.466-11.382.048L.875 37.958c-2.746-4.814 1.371-10.646 6.827-9.67l120.385 21.517a6.537 6.537 0 0 0 2.322-.004l117.867-21.483c5.438-.991 9.574 4.796 6.877 9.62Z"></path><path fill="url(#IconifyId1813088fe1fbc01fb467)" d="M185.432.063L96.44 17.501a3.268 3.268 0 0 0-2.634 3.014l-5.474 92.456a3.268 3.268 0 0 0 3.997 3.378l24.777-5.718c2.318-.535 4.413 1.507 3.936 3.838l-7.361 36.047c-.495 2.426 1.782 4.5 4.151 3.78l15.304-4.649c2.372-.72 4.652 1.36 4.15 3.788l-11.698 56.621c-.732 3.542 3.979 5.473 5.943 2.437l1.313-2.028l72.516-144.72c1.215-2.423-.88-5.186-3.54-4.672l-25.505 4.922c-2.396.462-4.435-1.77-3.759-4.114l16.646-57.705c.677-2.35-1.37-4.583-3.769-4.113Z"></path></svg>
\ No newline at end of file
diff --git a/embryo-frontend/src/App.tsx b/embryo-frontend/src/App.tsx
new file mode 100644
index 0000000..eae7be9
--- /dev/null
+++ b/embryo-frontend/src/App.tsx
@@ -0,0 +1,15 @@
+import React from "react";
+import { BrowserRouter as Router, Routes, Route } from "react-router-dom";
+import GeneView from "./pages/GeneView";
+
+function App() {
+  return (
+    <Router>
+      <Routes>
+        <Route path="/" element={<GeneView />} />
+      </Routes>
+    </Router>
+  );
+}
+
+export default App;
diff --git a/embryo-frontend/src/api/geneAPI.ts b/embryo-frontend/src/api/geneAPI.ts
new file mode 100644
index 0000000..ce09481
--- /dev/null
+++ b/embryo-frontend/src/api/geneAPI.ts
@@ -0,0 +1,6 @@
+import axios from "axios";
+
+export const fetchGeneExpression = async (gene: string) => {
+  const res = await axios.get(`http://localhost:5000/api/gene/${gene}`);
+  return res.data;
+};
\ No newline at end of file
diff --git a/embryo-frontend/src/components/PointCloud.tsx b/embryo-frontend/src/components/PointCloud.tsx
new file mode 100644
index 0000000..9e47d96
--- /dev/null
+++ b/embryo-frontend/src/components/PointCloud.tsx
@@ -0,0 +1,129 @@
+import React, { useEffect, useRef } from "react";
+import * as THREE from "three";
+import { OrbitControls } from "three/examples/jsm/controls/OrbitControls";
+
+interface Point {
+  x: number;
+  y: number;
+  z: number;
+  value: number; // 表达强度
+}
+
+interface PointCloudProps {
+  data: Point[];
+}
+
+const PointCloud: React.FC<PointCloudProps> = ({ data }) => {
+  const containerRef = useRef<HTMLDivElement | null>(null);
+  const rendererRef = useRef<THREE.WebGLRenderer>();
+
+  useEffect(() => {
+    if (!containerRef.current || data.length === 0) return;
+
+    // 清除旧渲染内容
+    if (containerRef.current.firstChild) {
+      containerRef.current.removeChild(containerRef.current.firstChild);
+    }
+
+    const width = containerRef.current.clientWidth;
+    const height = containerRef.current.clientHeight;
+
+    // 初始化场景、相机、渲染器
+    const scene = new THREE.Scene();
+    const camera = new THREE.PerspectiveCamera(60, width / height, 0.1, 1000);
+    camera.position.set(0, 0, 30);
+
+    const renderer = new THREE.WebGLRenderer({ antialias: true });
+    renderer.setSize(width, height);
+    containerRef.current.appendChild(renderer.domElement);
+
+    // 控件
+    const controls = new OrbitControls(camera, renderer.domElement);
+    controls.enableDamping = true;
+
+    // 坐标轴辅助
+    const axesHelper = new THREE.AxesHelper(10);
+    scene.add(axesHelper);
+
+    // 点云数据
+    const geometry = new THREE.BufferGeometry();
+    const positions: number[] = [];
+    const colors: number[] = [];
+    const sizes: number[] = [];
+
+    const color = new THREE.Color();
+    const maxValue = Math.max(...data.map((p) => p.value));
+
+    data.forEach((point) => {
+      positions.push(point.x, point.y, point.z);
+      color.setHSL(0.6 * (1 - point.value / maxValue), 1.0, 0.5);
+      colors.push(color.r, color.g, color.b);
+      sizes.push(0.3 + 1.5 * point.value / maxValue); // 点大小映射
+    });
+
+    geometry.setAttribute("position", new THREE.Float32BufferAttribute(positions, 3));
+    geometry.setAttribute("color", new THREE.Float32BufferAttribute(colors, 3));
+    geometry.setAttribute("size", new THREE.Float32BufferAttribute(sizes, 1));
+
+    const material = new THREE.PointsMaterial({
+      vertexColors: true,
+      size: 0.5,
+      sizeAttenuation: true,
+    });
+
+    const points = new THREE.Points(geometry, material);
+    scene.add(points);
+
+    // 点击点检测（raycaster）
+    const raycaster = new THREE.Raycaster();
+    const mouse = new THREE.Vector2();
+
+    const onClick = (event: MouseEvent) => {
+      const rect = renderer.domElement.getBoundingClientRect();
+      mouse.x = ((event.clientX - rect.left) / rect.width) * 2 - 1;
+      mouse.y = -((event.clientY - rect.top) / rect.height) * 2 + 1;
+
+      raycaster.setFromCamera(mouse, camera);
+      const intersects = raycaster.intersectObject(points);
+
+      if (intersects.length > 0) {
+        const i = intersects[0].index ?? 0;
+        const p = data[i];
+        console.log(`Clicked: x=${p.x}, y=${p.y}, z=${p.z}, value=${p.value}`);
+        alert(`Cell @ (${p.x.toFixed(1)}, ${p.y.toFixed(1)}, ${p.z.toFixed(1)})\nExpression: ${p.value.toFixed(3)}`);
+      }
+    };
+
+    renderer.domElement.addEventListener("click", onClick);
+
+    // 渲染循环
+    const animate = () => {
+      requestAnimationFrame(animate);
+      controls.update();
+      renderer.render(scene, camera);
+    };
+    animate();
+
+    // 图例（颜色条）
+    const legend = document.createElement("div");
+    legend.innerHTML = `
+      <div style="position:absolute;top:10px;right:10px;background:white;padding:5px;border:1px solid gray;font-size:12px;">
+        <div style="background:linear-gradient(to top, hsla(180, 100%, 50%, 1.00),hsla(72, 100%, 50%, 1.00), hsla(0, 100%, 50%, 0.99)); width:20px; height:100px;"></div>
+        <div>↑ High</div>
+        <div>↓ Low</div>
+      </div>`;
+    containerRef.current.appendChild(legend);
+
+    // 清理
+    return () => {
+      renderer.dispose();
+      geometry.dispose();
+      material.dispose();
+      renderer.domElement.removeEventListener("click", onClick);
+    };
+  }, [data]);
+
+  return <div ref={containerRef} style={{ width: "100%", height: "100%", position: "relative" }} />;
+};
+
+export default PointCloud;
diff --git a/embryo-frontend/src/main.tsx b/embryo-frontend/src/main.tsx
new file mode 100644
index 0000000..11c2536
--- /dev/null
+++ b/embryo-frontend/src/main.tsx
@@ -0,0 +1,10 @@
+import React from 'react'
+import ReactDOM from 'react-dom/client'
+import App from './App'
+import './style.css'
+
+ReactDOM.createRoot(document.getElementById('root')!).render(
+  <React.StrictMode>
+    <App />
+  </React.StrictMode>,
+)
\ No newline at end of file
diff --git a/embryo-frontend/src/pages/GeneView.tsx b/embryo-frontend/src/pages/GeneView.tsx
new file mode 100644
index 0000000..31df47b
--- /dev/null
+++ b/embryo-frontend/src/pages/GeneView.tsx
@@ -0,0 +1,122 @@
+import React, { useState, useEffect } from "react";
+import axios from "axios";
+import PointCloud from "../components/PointCloud";
+
+const GeneView: React.FC = () => {
+  const [gene, setGene] = useState("");
+  const [data, setData] = useState<any[]>([]);
+  const [loading, setLoading] = useState(false);
+  const [error, setError] = useState("");
+  const [availableGenes, setAvailableGenes] = useState<string[]>([]);
+  const [selectedStage, setSelectedStage] = useState("CS7");
+
+  // 动态获取某个阶段的基因列表
+  useEffect(() => {
+    if (!selectedStage) return;
+    axios
+      .get("http://localhost:5000/api/genes", {
+        params: { stage: selectedStage },
+      })
+      .then((res) => setAvailableGenes(res.data))
+      .catch(() => setAvailableGenes([]));
+  }, [selectedStage]);
+
+  const handleSearch = async () => {
+    if (!gene) return;
+
+    setLoading(true);
+    setError("");
+    setData([]);
+
+    try {
+      const res = await axios.get("http://localhost:5000/api/gene_expression", {
+        params: {
+          gene: gene.trim(),
+          stage: selectedStage,
+        },
+      });
+
+      if (res.data.expression.length === 0) {
+        setError("No expression data found.");
+      } else {
+        setData(res.data.expression);
+      }
+    } catch (err: any) {
+      if (err.response && err.response.data?.error) {
+        setError(err.response.data.error);
+      } else {
+        setError("Failed to fetch data.");
+      }
+    } finally {
+      setLoading(false);
+    }
+  };
+
+  return (
+    <div style={{ padding: "1rem" }}>
+      <h2>Gene Expression Viewer</h2>
+
+      {/* 阶段选择 */}
+      <div style={{ marginBottom: "10px" }}>
+        <label>
+          Stage:&nbsp;
+          <select
+            value={selectedStage}
+            onChange={(e) => {
+              setSelectedStage(e.target.value);
+              setGene("");
+              setData([]);
+              setError("");
+            }}
+          >
+            <option value="CS7">CS7</option>
+            <option value="CS8">CS8</option>
+            <option value="CS9">CS9</option>
+          </select>
+        </label>
+      </div>
+
+      {/* 基因选择 + 按钮 */}
+      <div style={{ marginBottom: "1rem" }}>
+        <select
+          value={gene}
+          onChange={(e) => setGene(e.target.value)}
+          style={{ marginRight: "1rem" }}
+        >
+          <option value="">-- Select a gene --</option>
+          {availableGenes.map((g) => (
+            <option key={g} value={g}>
+              {g}
+            </option>
+          ))}
+        </select>
+        <button onClick={handleSearch} disabled={!gene || loading}>
+          {loading ? "Loading..." : "Search"}
+        </button>
+      </div>
+
+      {/* 错误提示 */}
+      {error && <p style={{ color: "red" }}>{error}</p>}
+
+      {/* 可视化区域 */}
+      <div
+        style={{
+          width: "100%",
+          height: "600px",
+          border: "1px solid #ccc",
+          position: "relative",
+        }}
+      >
+        {data.length > 0 ? (
+          <PointCloud data={data} />
+        ) : (
+          <p style={{ textAlign: "center", paddingTop: "2rem" }}>
+            No data to display.
+          </p>
+        )}
+      </div>
+    </div>
+  );
+};
+
+export default GeneView;
diff --git a/embryo-frontend/src/style.css b/embryo-frontend/src/style.css
new file mode 100644
index 0000000..3bcdbd0
--- /dev/null
+++ b/embryo-frontend/src/style.css
@@ -0,0 +1,96 @@
+:root {
+  font-family: system-ui, Avenir, Helvetica, Arial, sans-serif;
+  line-height: 1.5;
+  font-weight: 400;
+
+  color-scheme: light dark;
+  color: rgba(255, 255, 255, 0.87);
+  background-color: #242424;
+
+  font-synthesis: none;
+  text-rendering: optimizeLegibility;
+  -webkit-font-smoothing: antialiased;
+  -moz-osx-font-smoothing: grayscale;
+}
+
+a {
+  font-weight: 500;
+  color: #646cff;
+  text-decoration: inherit;
+}
+a:hover {
+  color: #535bf2;
+}
+
+body {
+  margin: 0;
+  display: flex;
+  place-items: center;
+  min-width: 320px;
+  min-height: 100vh;
+}
+
+h1 {
+  font-size: 3.2em;
+  line-height: 1.1;
+}
+
+#app {
+  max-width: 1280px;
+  margin: 0 auto;
+  padding: 2rem;
+  text-align: center;
+}
+
+.logo {
+  height: 6em;
+  padding: 1.5em;
+  will-change: filter;
+  transition: filter 300ms;
+}
+.logo:hover {
+  filter: drop-shadow(0 0 2em #646cffaa);
+}
+.logo.vanilla:hover {
+  filter: drop-shadow(0 0 2em #3178c6aa);
+}
+
+.card {
+  padding: 2em;
+}
+
+.read-the-docs {
+  color: #888;
+}
+
+button {
+  border-radius: 8px;
+  border: 1px solid transparent;
+  padding: 0.6em 1.2em;
+  font-size: 1em;
+  font-weight: 500;
+  font-family: inherit;
+  background-color: #1a1a1a;
+  cursor: pointer;
+  transition: border-color 0.25s;
+}
+button:hover {
+  border-color: #646cff;
+}
+button:focus,
+button:focus-visible {
+  outline: 4px auto -webkit-focus-ring-color;
+}
+
+@media (prefers-color-scheme: light) {
+  :root {
+    color: #213547;
+    background-color: #ffffff;
+  }
+  a:hover {
+    color: #747bff;
+  }
+  button {
+    background-color: #f9f9f9;
+  }
+}
diff --git a/embryo-frontend/src/typescript.svg b/embryo-frontend/src/typescript.svg
new file mode 100644
index 0000000..d91c910
--- /dev/null
+++ b/embryo-frontend/src/typescript.svg
@@ -0,0 +1 @@
+<svg xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" aria-hidden="true" role="img" class="iconify iconify--logos" width="32" height="32" preserveAspectRatio="xMidYMid meet" viewBox="0 0 256 256"><path fill="#007ACC" d="M0 128v128h256V0H0z"></path><path fill="#FFF" d="m56.612 128.85l-.081 10.483h33.32v94.68h23.568v-94.68h33.321v-10.28c0-5.69-.122-10.444-.284-10.566c-.122-.162-20.4-.244-44.983-.203l-44.74.122l-.121 10.443Zm149.955-10.742c6.501 1.625 11.459 4.51 16.01 9.224c2.357 2.52 5.851 7.111 6.136 8.208c.08.325-11.053 7.802-17.798 11.988c-.244.162-1.22-.894-2.317-2.52c-3.291-4.795-6.745-6.867-12.028-7.233c-7.76-.528-12.759 3.535-12.718 10.321c0 1.992.284 3.17 1.097 4.795c1.707 3.536 4.876 5.649 14.832 9.956c18.326 7.883 26.168 13.084 31.045 20.48c5.445 8.249 6.664 21.415 2.966 31.208c-4.063 10.646-14.14 17.879-28.323 20.276c-4.388.772-14.79.65-19.504-.203c-10.28-1.828-20.033-6.908-26.047-13.572c-2.357-2.6-6.949-9.387-6.664-9.874c.122-.163 1.178-.813 2.356-1.504c1.138-.65 5.446-3.129 9.509-5.485l7.355-4.267l1.544 2.276c2.154 3.29 6.867 7.801 9.712 9.305c8.167 4.307 19.383 3.698 24.909-1.26c2.357-2.153 3.332-4.388 3.332-7.68c0-2.966-.366-4.266-1.91-6.501c-1.99-2.845-6.054-5.242-17.595-10.24c-13.206-5.69-18.895-9.224-24.096-14.832c-3.007-3.25-5.852-8.452-7.03-12.8c-.975-3.617-1.22-12.678-.447-16.335c2.723-12.76 12.353-21.659 26.25-24.3c4.51-.853 14.994-.528 19.424.569Z"></path></svg>
\ No newline at end of file
diff --git a/embryo-frontend/src/vite-env.d.ts b/embryo-frontend/src/vite-env.d.ts
new file mode 100644
index 0000000..11f02fe
--- /dev/null
+++ b/embryo-frontend/src/vite-env.d.ts
@@ -0,0 +1 @@
+/// <reference types="vite/client" />
diff --git a/embryo-frontend/tsconfig.json b/embryo-frontend/tsconfig.json
new file mode 100644
index 0000000..9cc289d
--- /dev/null
+++ b/embryo-frontend/tsconfig.json
@@ -0,0 +1,26 @@
+{
+  "compilerOptions": {
+    "jsx": "react",
+    "target": "ES2022",
+    "useDefineForClassFields": true,
+    "module": "ESNext",
+    "lib": ["ES2022", "DOM", "DOM.Iterable"],
+    "skipLibCheck": true,
+
+    /* Bundler mode */
+    "moduleResolution": "bundler",
+    "allowImportingTsExtensions": true,
+    "verbatimModuleSyntax": true,
+    "moduleDetection": "force",
+    "noEmit": true,
+
+    /* Linting */
+    "strict": true,
+    "noUnusedLocals": true,
+    "noUnusedParameters": true,
+    "erasableSyntaxOnly": true,
+    "noFallthroughCasesInSwitch": true,
+    "noUncheckedSideEffectImports": true
+  },
+  "include": ["src"]
+}
diff --git a/pyproject.toml b/pyproject.toml
new file mode 100644
index 0000000..27c2fdb
--- /dev/null
+++ b/pyproject.toml
@@ -0,0 +1,12 @@
+[project]
+name = "digital-embryo"
+version = "0.1.0"
+description = "Add your description here"
+readme = "README.md"
+requires-python = ">=3.13"
+dependencies = [
+    "anndata>=0.12.1",
+    "flask>=3.1.1",
+    "flask-cors>=6.0.1",
+    "pandas>=2.3.1",
+]
diff --git a/start-dev.ps1 b/start-dev.ps1
new file mode 100644
index 0000000..1bb1bf3
--- /dev/null
+++ b/start-dev.ps1
@@ -0,0 +1,107 @@
+#!/usr/bin/env pwsh
+
+# 启动开发环境脚本
+Write-Host "正在启动 Digital Embryo 开发环境..." -ForegroundColor Green
+
+# 获取当前脚本所在目录
+$ScriptDir = Split-Path -Parent $MyInvocation.MyCommand.Path
+
+# 前端目录路径
+$FrontendDir = Join-Path $ScriptDir "embryo-frontend"
+# 后端目录路径
+$BackendDir = Join-Path $ScriptDir "embryo-backend"
+
+# 检查目录是否存在
+if (-not (Test-Path $FrontendDir)) {
+    Write-Error "前端目录不存在: $FrontendDir"
+    exit 1
+}
+
+if (-not (Test-Path $BackendDir)) {
+    Write-Error "后端目录不存在: $BackendDir"
+    exit 1
+}
+
+# 检查必要文件是否存在
+$PackageJsonPath = Join-Path $FrontendDir "package.json"
+$AppPyPath = Join-Path $BackendDir "app.py"
+
+if (-not (Test-Path $PackageJsonPath)) {
+    Write-Error "package.json 文件不存在: $PackageJsonPath"
+    exit 1
+}
+
+if (-not (Test-Path $AppPyPath)) {
+    Write-Error "app.py 文件不存在: $AppPyPath"
+    exit 1
+}
+
+Write-Host "启动前端开发服务器..." -ForegroundColor Yellow
+# 启动前端开发服务器
+$FrontendJob = Start-Job -ScriptBlock {
+    param($FrontendDir)
+    Set-Location $FrontendDir
+    npm run dev
+} -ArgumentList $FrontendDir
+
+Write-Host "启动后端API服务器..." -ForegroundColor Yellow
+# 启动后端API服务器
+$BackendJob = Start-Job -ScriptBlock {
+    param($ScriptDir, $BackendDir)
+    Set-Location $ScriptDir
+    uv run $BackendDir/app.py
+} -ArgumentList $ScriptDir, $BackendDir
+
+Write-Host "两个服务都已启动!" -ForegroundColor Green
+Write-Host "前端开发服务器通常运行在: http://localhost:5173" -ForegroundColor Cyan
+Write-Host "后端API服务器通常运行在: http://localhost:5000" -ForegroundColor Cyan
+Write-Host ""
+Write-Host "按 Ctrl+C 停止所有服务" -ForegroundColor Red
+
+# 等待任意作业完成或用户中断
+try {
+    # 持续监控作业状态
+    while ($true) {
+        $FrontendState = Get-Job -Id $FrontendJob.Id | Select-Object -ExpandProperty State
+        $BackendState = Get-Job -Id $BackendJob.Id | Select-Object -ExpandProperty State
+
+        # 检查是否有作业失败
+        if ($FrontendState -eq "Failed") {
+            Write-Error "前端服务启动失败"
+            Receive-Job -Id $FrontendJob.Id
+            break
+        }
+
+        if ($BackendState -eq "Failed") {
+            Write-Error "后端服务启动失败"
+            Receive-Job -Id $BackendJob.Id
+            break
+        }
+
+        # 如果两个作业都完成了（正常或异常），退出循环
+        if ($FrontendState -eq "Completed" -or $BackendState -eq "Completed") {
+            break
+        }
+
+        Start-Sleep -Seconds 1
+    }
+}
+catch {
+    Write-Host "接收到中断信号，正在停止服务..." -ForegroundColor Yellow
+}
+finally {
+    # 清理作业
+    Write-Host "正在停止所有服务..." -ForegroundColor Yellow
+
+    if ($FrontendJob) {
+        Stop-Job -Id $FrontendJob.Id -ErrorAction SilentlyContinue
+        Remove-Job -Id $FrontendJob.Id -Force -ErrorAction SilentlyContinue
+    }
+
+    if ($BackendJob) {
+        Stop-Job -Id $BackendJob.Id -ErrorAction SilentlyContinue
+        Remove-Job -Id $BackendJob.Id -Force -ErrorAction SilentlyContinue
+    }
+
+    Write-Host "所有服务已停止" -ForegroundColor Green
+}
\ No newline at end of file
diff --git a/uv.lock b/uv.lock
new file mode 100644
index 0000000..7c01d4d
--- /dev/null
+++ b/uv.lock
@@ -0,0 +1,468 @@
+version = 1
+revision = 2
+requires-python = ">=3.13"
+
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+version = "0.12.1"
+source = { registry = "https://pypi.org/simple" }
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+    { name = "array-api-compat" },
+    { name = "h5py" },
+    { name = "legacy-api-wrap" },
+    { name = "natsort" },
+    { name = "numpy" },
+    { name = "packaging" },
+    { name = "pandas" },
+    { name = "scipy" },
+    { name = "zarr" },
+]
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