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> Here we have analysed stem cell-derived cerebral organoids using single-cell transcriptomics and accessible chromatin profiling to investigate gene-regulatory changes that are specific to humans. Our data provide a temporal cell atlas of great ape forebrain development, and illuminate dynamic gene-regulatory features that are unique to humans. > Here we have analysed stem cell-derived cerebral organoids using single-cell transcriptomics and accessible chromatin profiling to investigate gene-regulatory changes that are specific to humans. Our data provide a temporal cell atlas of great ape forebrain development, and illuminate dynamic gene-regulatory features that are unique to humans.
5. [人CS8时期的空间转录组数据分析](https://cs8.3dembryo.com/) 5. [**人CS8时期的空间转录组数据分析**](https://cs8.3dembryo.com/)
> This database is intended to curate 3D spatial transcriptomes of a human gastrulating embryo at Carnegie stage(CS) 8, equivalent to 18-19 days post-fertilization by Stereo-seq. For currently available data, the human CS8 embryo was collected and subjected to cryosection to generate 10 μm thick slices from the rostral side to the caudal side. All slices were applied to Stereo-seq chips to capture their 2D spatial transcriptomes. All the 2D spatial transcriptomes were combined to recreate their 3D spatial transcriptomes and reconstruction 3D CS8 human embryo model. With these data, one could visualize and analyze spatial expression patterns of genes of interest, 3D reconstruct cluster-specific spatial transcriptomes by clustering and annotation, identify cell signaling pathways and gene regulatory networks, examine gene functions in their intact spatial context, provide a unique opportunity to explore the critical cellular and molecular features of gastrulating events, etc. > This database is intended to curate 3D spatial transcriptomes of a human gastrulating embryo at Carnegie stage(CS) 8, equivalent to 18-19 days post-fertilization by Stereo-seq. For currently available data, the human CS8 embryo was collected and subjected to cryosection to generate 10 μm thick slices from the rostral side to the caudal side. All slices were applied to Stereo-seq chips to capture their 2D spatial transcriptomes. All the 2D spatial transcriptomes were combined to recreate their 3D spatial transcriptomes and reconstruction 3D CS8 human embryo model. With these data, one could visualize and analyze spatial expression patterns of genes of interest, 3D reconstruct cluster-specific spatial transcriptomes by clustering and annotation, identify cell signaling pathways and gene regulatory networks, examine gene functions in their intact spatial context, provide a unique opportunity to explore the critical cellular and molecular features of gastrulating events, etc.
> Xiao Z, Cui L, Yuan Y, He N, Xie X, Lin S, Yang X, Zhang X, Shi P, Wei Z, Li Y, Wang H, Wang X, Wei Y, Guo J, Yu L. 3D reconstruction of a gastrulating human embryo. Cell. 2024 May 23;187(11):2855-2874.e19. doi: 10.1016/j.cell.2024.03.041. > Xiao Z, Cui L, Yuan Y, He N, Xie X, Lin S, Yang X, Zhang X, Shi P, Wei Z, Li Y, Wang H, Wang X, Wei Y, Guo J, Yu L. 3D reconstruction of a gastrulating human embryo. Cell. 2024 May 23;187(11):2855-2874.e19. doi: 10.1016/j.cell.2024.03.041.
6. [**HDBR Atlas**](https://hdbratlas.org/copyright.html)
> The HDBR Atlas is a unique resource which aims to facilitate the understanding of the development of the human embryo and fetus.
> The atlas began as the Electronic Atlas of the Developing Human Brain (EADHB), which was funded by the US National Institutes of Health (NIH) Human Brain Project (grant numbers: HD39928-02 followed by R01 MH070370).
> The long-term aim of this work was to create a digital atlas comprising 3D reconstructions from Carnegie Stage 12 to 23, generated using Optical Projection Tomography (OPT; Sharpe et al 2002), and annotations of the 3D models linked to an anatomical database.
> The digital atlas was also linked to a gene expression database that was developed from the Edinburgh Mouse Atlas Project gene expression database (EMAGE). Again, the major funder was NIH, as above, with additional funding from EU FP6 (DGEMap, contract number 011993).
> With the agreement of the funders, both the digital atlas and the gene expression database were brought together on the HuDSeN website and became the HuDSeN Electronic Atlas of the Developing Human and HuDSeN Human Gene Expression Spatial Database.
> Currently the MRC/Wellcome Trust-funded HDBR project is supporting the atlas and gene expression database, and the name has been changed to the HDBR Atlas to reflect this.
> In the future, the HDBR Atlas aims to provide the wider scientific, educational and medical communities with a dynamic tool for documenting and analyzing gene expression patterns and morphological changes during human embryonic and fetal development.