更新项目参考网站

2025-07-26 11:55:51 +08:00 · 2025-07-26 11:55:51 +08:00 · e26077f20b
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 > MOSTA database has a total of 53 sagittal sections from C57BL/6 mouse embryos at E9.5 (~7.1 mm2), E10.5 (~11.5 mm2), E11.5 (~18.8 mm2), E12.5 (~32.1 mm2), E13.5 (~48.4 mm2), E14.5 (~64.1 mm2), E15.5 (~70.8 mm2) and E16.5 (~76.1 mm2) using Stereo-seq. For E9.5-E15.5 stages, four to six sections were included from different replicates. As for E16.5, 17 sagittal sections were profiled from two biological replicates, with 13 sections from one single embryo, allowing coverage of all major organs/tissues. In the MOSTA, we provide the spatial map showing the gene expression, gene co-expression modules and regulons in each embryo sagittal sections. Our panoramic atlas will allow in-depth investigation of longstanding questions concerning mammalian development.

-3. 人CS8时期的空间转录组数据分析平台
+3. [斑马鱼早期胚胎形成的空间转录组数据分析平台](https://db.cngb.org/stomics/zesta/)
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+> Vertebrate embryogenesis is a remarkably dynamic process during which numerous cell types of different lineages generate, change, or disappear within a short period. A major challenge in understanding this process is the lack of topographical transcriptomic information that can help correlate microenvironmental cues within the hierarchy of cell fate decisions. Here, we employed Stereo-seq to dissect the spatiotemporal dynamics of gene expression and regulatory networks in the developing zebrafish embryos. We profiled 91 embryo sections covering six critical time points during the first 24 hours of development, obtaining a total of 152,977 spots at a resolution of 10x10x15 µm3 (close to cellular size) with spatial coordinates. Meanwhile, we identified spatial modules and co-varying genes for specific tissue organizations. By performing the integrated analysis of the Stereo-seq and scRNA-seq data from each time point, we reconstructed the spatially resolved developmental trajectories of cell fate transitions and molecular changes during zebrafish embryogenesis. Our study constitutes a fundamental reference for further studies aiming to understand vertebrate development.